CRISPR-Cas9

CRISPR/Cas9-based genome editing enables rapid genetic manipulation of any genomic locus. CRISPR/Cas9 was originally employed to “knock-out/Knock-in” target genes, modifications to the Cas9 enzyme have allowed the application of CRISPR to selectively activate or repress target genes.

The CRISPR/Cas9 system consists of two major components: a specific “guide” RNA (gRNA) and a non-specific CRISPR-associated endonuclease (Cas9). The gRNA is a short synthetic RNA composed of a “scaffold” sequence necessary for Cas9-binding and a ∼20 nucleotide “targeting” sequence which defines the genomic target to be modified. 

To achieve efficient gene editing, Cas9 has to be expressed to certain levels in target cells. It is critical to select a suitable promoter to drive Cas9 expression. Cellomics Technology has premade lentivirus with various promoters driven Cas9 with or without GFP/RFP fluorescent marker. The lentivirus has puromycin or neomycin selection marker for selecting Cas9 stable cell clones. We also developed tet inducible Cas9 lentivirus, for turning on Cas9 expression only at certain time. 

To facilitate your research, Cellomics Technology provide a collection of CRISPR-Cas9 premade lentivirus ready for your gene editing need, and CRISPR-Cas9 libraries for specific pathway.