EliteTrust Custom Lentivirus Production

Cellomics Technology, LLC provides customers with flexible and best quality lentiviral vector production services, from construct design, to lentivral vector production, concentration and titration. 

Lentiviral vector is a powerful tool for delivering target genes into almost all types of dividing and non-dividing mammalian cells in vitro and in vivo. However, production of recombinant lentiviral vector is laborious and challenging, especially when high titer of recombinant lentiviral vectors (>107 transducing unit (TU)/ml) is required. 

We have big collection of premade Ready-To-Use lentiviruses to meet general purposes. Please check out our  Pre-made lentivirus first, to see if we have ready-to-use lentivirus for your purpose.   We also provide custom services to make lentivirus for you if no lentivirus is available to meet you need. 

Our standardized and optimized lentiviral vector production lines and strict quality control systems enables us to generate the best quality of recombinant lentivirus for our customers in shortest turnaround time.

Our unique lentivirus concentration reagent ViroGold and purification technologies set strong foundation for us to produce high purity lentivirus. The final lentivirus delivered to customers is preserved in LentiGuard reagent, invented by scientists of Cellomics Technology, LLC, keeping your experiments consistent and reliable all the time. 

Finally, all the lentivirus are titrated with functional assay, rather than physical titration assay, such as p24 ELISA, or lentivirus genome copy number in stock determined with qPCR. The functional titer is the best reference to calculate how much lentivirus stock need in target cell transduction experiment.  

Advantages of our custom lentivirus production service:

1. Shortest Turnaround time;

2. Preserved in LentiGuard; 

3. Functional titration;

4. High purity and low cytotoxicity for in vitro and in vivo; 

Notes:

1. Lentiviral vector titer by functional unit is determined by Cellomics Technology, LLC, that is equivalent to more than 5 times higher than that determined by physical particle titration, such as p24, conventional qPCR, etc. 
   
2. Our unique concentration and purification technologies using ViroGold greatly enhances lentivirus purity  by getting rid of host proteins from packaging cell lines.  That makes sure the delivered lentivirus in high purity and low cytotoxicity. 
3. All lentiiviruses are preserved in LentiGuard, special reagent invented by scientists of Cellomics Technology, LLC, that will keep lentivirus in high quality and high titer all the time, by protecting lentivirus from damage resulted from freeze-thaw cycles. 
   
4. One unit of lentivirus from Cellomics Technology, LLC is roughly equivalent to more than 5 units of IFU/ml or TU/ml from our competitor.
5.  Turn-around Time: 1-2 weeks.  
6. For questions and/or order, please email [email protected].

Notes:

• * The Crude means that the lentivirus is not purified, however the lentivirus was produced in the medium without fetal bovine serum (FBS). The virus works well with many different cell types.
• ** The lentivirus is concentrated and purified after harvested from the medium without FBS, these lentivirus shows no cytotoxicity to most of human or mouse cells.
• ***Ultra Pure lentivirus is produced, concentrated and double purified in the medium without FBS, the double purification means that lentivirus goes through two different purification methods to reach ultra high purity, the lentivirus has no noticeable cytotoxicity even to very sensitive cell types like primary T lymphocytes, NK cells or other special cell types.
• # The lentivirus is designed for the experiments that require to deliver extremely high quantity of lentivirus in extramely small volume, for example, mouse eye or mouse brain injection. The lentivirus is ultra pure, and ultra high titer to prevent toxicity and deformation of organ injected.
• Descriptions:
  1. CLVP-2E6-C1500: Small Scale of Lentivirus Production.
  2. CLVP-1E7-P1000: Enhanced-Titer Lentivirus Production & Purification.
  3. CLVP-1E8-P500: Basic Scale of Standard-Titer Lentivirus Production & Purification.
  4. CLVP-1E8-P1000: Standard Scale of Standard-Titer Lentivirus Production & Purification
  5. CLVP-1E9-P200: Basic Scale of Premium-Titer Lentivirus Production & Purification.
  6. CLVP-1E9-P500: Standard Scale of Premium-Titer lentivirus Production & Purification.
  7. CLVP-1E9-P1000: Large Scale of Premium-Titer lentivirus Production & Purification.
  8. CLVP-1E9-EXP200: Small Scale of High Titer Lentivirus Production & Purification.
  9. CLVP-1E9-EXP500: Standard Scale of High Titer Lentivirus Production & Purification.
  10. CLVP-1E9-EXP1000: Large Scale of High Titer Lentivirus Production & Purification.
  11. CLVP-1E10-EXP50#: Standard Scale of Ultra-High Titer Lentivirus Production & Purification.
  12. CLVP-1E10-EXP100#: Large Scale of Ultra-High Titer Lentivirus Production & Purification.
  13. CLVP-EX-Quote: Custom Scale.

How custom lentivirus production service proceeds:

If lentiviral construct is ready, please provide us:

1. The construct map or sequence. 

2.1~5 µg of your lentiviral construct plasmid DNA or bacterial culture.

We will prepare Endotoxin-free plasmid and transfect our packaging cell line for your lentivirus production. Lentiviral particles will be harvested followed by concentration and titration.

For 200 ul of lentivirus production,  You will receive eight  25 µl aliquots.

If you need us to generate lentiviral transfer vector for your target gene, please:

1. Choose lentiviral vector format from our vector collection and specify the gene of interest.

2. Please send us 1~5 µg plasmid or bacterial culture for your gene if it is available, otherwise please send us cDNA sequence.

We will subclone the gene of interest or shRNA into the chosen lentiviral vector and confirm its accuracy by sequencing.


To get a custom quote, please complete the Request a Quote Form below.