Lentivirus Concentration and Purification kit

Lentivirus concentration is one of the critical factors to which the titer and purity of final lentivirus stock is subjected. Unsuccessful concentration of lentivirus results in impure and low titer of lentivirus.

Commonly used lentivirus concentration methods, such as ultrafiltration and ultracentrifugation, are tedious and labor-consuming, resulting in impure lentivirus stock with lots of cell debris and protein contaminates from cell culture medium. The pellet from ultracentrifugation is big and dense, hard to dissolve by vigorous vortexing or pipetting, even soaking overnight does not help much. Polyethylene Glycol (PEG) precipitation is another common used method to concentrate lentivirus, the lentivirus concentration can be done in low centrifuge speed, but it is impossible to concentrate the virus over hundred folds and the recovery rate is frequently low.  

To overcome these challenges, scientists of Cellomics technology developed an unique and easy-to-use lentivirus concentration and purification kit ViroGold Lentivirus Concentrator. ViroGold Lentivirus Concentrator can concentrate lentivirus over thousands folds and result in ultrapure lentiviral particles, ready for in vitro and in vivo applications.The ViroGold saves you significant amount of time on virus concentration, the total manual operation takes less than 10 minutes. All you need to do is to add ViroGold to your lentiviral supernatant in centrifuge tube, centrifuge overnight, discard the supernatant, dissolve small pure virus pellet. The lentivirus pellet from ViroGold is very easy to be dissolved in two minutes. It is straight forward to scale up ViroGold lentivirus Concentrator to concentrate liters of lentiviral supernatant. 

After concentration, the lentivirus stock can be stored in Lentivirus Freeze-Thaw Protection Medium LentiGuard to prevent lentivirus titer loss during the freeze-thaw cycles.