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- CRISPR-Cas9
- Cell Culture and Transfection Reagents
- EliteTrust Human Gene ORF Virus Particle Collection
- Featured Products
- Lentiviral Cloning Vectors
- Lentivirus Concentration and Purification kit
- Lentivirus Freeze-Thaw Protection Medium
- Lentivirus Packaging System
- Lentivirus Titration
- Premade Lentivirus
- Premade Stable Cell Line
- Stem Cell Research
- Vectors for iPS Cells Generation
- xpath of 1st category mapping
- xpath of 2nd category mapping
- DNA Mutagenesis
- EliteTrust Cell Authentication Service
- EliteTrust Custom Adeno-Associated Virus Production
- EliteTrust Custom Gene Targeting Service
- EliteTrust Custom Lentivirus Production
- EliteTrust Custom Retrovirus Production Service
- EliteTrust Custom Stable Cell Line Generation Service
- EliteTrust Stable Cell Line Mycoplasma Detection Service
Cas 9 lentivirus
CRISPR-Cas9 technology has been widely used for gene editing. To achieve high efficient gene editing, the optimal Cas9 endonuclease expression level is ciritical. Promoter activity varies in different cell types. Cas9 lentiviruses express Cas9 under different constitutive promoters or tet-inducible promoter, providing the flexibility to select a promoter to drive optimal Cas9 expression and achieve high gene editing efficiency. Tet-inducible Cas9 allows to express Cas9 only at certain period of time.