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- CRISPR-Cas9
- Cell Culture and Transfection Reagents
- EliteTrust Human Gene ORF Virus Particle Collection
- Featured Products
- Lentiviral Cloning Vectors
- Lentivirus Concentration and Purification kit
- Lentivirus Freeze-Thaw Protection Medium
- Lentivirus Packaging System
- Lentivirus Titration
- Premade Lentivirus
- Premade Stable Cell Line
- Stem Cell Research
- Vectors for iPS Cells Generation
- xpath of 1st category mapping
- xpath of 2nd category mapping
- DNA Mutagenesis
- EliteTrust Cell Authentication Service
- EliteTrust Custom Adeno-Associated Virus Production
- EliteTrust Custom Gene Targeting Service
- EliteTrust Custom Lentivirus Production
- EliteTrust Custom Retrovirus Production Service
- EliteTrust Custom Stable Cell Line Generation Service
- EliteTrust Stable Cell Line Mycoplasma Detection Service
R&D Lentivirus Prodution
Cellomics Technology, LLC provides customers with flexible and best quality lentiviral vector production services, from construct design, to lentivral vector production, concentration and titration.
Lentiviral vector is a powerful tool for delivering target genes into almost all types of dividing and non-dividing mammalian cells in vitro and in vivo. However, production of recombinant lentiviral vector is laborious and challenging, especially when high titer of recombinant lentiviral vectors (>107 transducing unit (TU)/ml) is required.
We manufacture both Custom and Ready-To-Use Lentiviral Vectors. Learn more about our Pre-made Lentiviral Vectors here.
Our standardized and optimized custom lentiviral vector production and strict quality control systems enables us to generate high titer of functional recombinant lentiviral vectors for our customers. Our titration procedures determine the quantity of functional lentiviral vectors rather than physical lentiviral particles.
| Cat. No. | Service Description | Vector Titer (TU/ml) |
Volume | Turnaround Time (weeks) |
Price (construct generation not included) |
CLVP-106-010ML |
Custom Crude Lentiviral Vector Production |
N/A |
10 ml |
2-3 |
$350 |
CLVP-107-001ML |
Custom Low-Titer of Lentiviral Vector Production |
>107 |
1 ml |
2-3 |
$1190 |
CLVP-108-200UL |
Custom Medium-Titer of Lentiviral Vector Production |
>108 |
200 µl |
2-3 |
$1590 |
CLVP-109-200UL |
Custom High-Titer of Lentiviral Vector Production |
>109 |
200 µl |
2-3 |
$2190 |
CLVP-110-020UL |
Custom Ultra-High-Titer of Lentiviral Vector Production |
>1010 |
20 µl |
2-3 |
$2490 |
CLVP-Quota |
Custom-Titer and Scale of Lentiviral Vector Production |
Custom |
Custom |
2-3 |
| Notes: * one titer unit of Cellomics Technology, LLC is roughly equivalent to more than 5 units of IFU/ml or TU/ml from our competitor. | ||||||
| ** Customer should provide enough amount of sterile, endotoxin-free ready-for-transfection transfer vector DNA for virus production. | ||||||
| *** Customer should provide enough amount of sterile, endotoxin-free ready-for-transfection transfer vector DNA for virus production. ****Titer is measured in clone formation unit (CFU) when antibiotics resistant gene is available in construct; titer in transducing units when fluorescence marker is available in construct; p24 ELISA or other assays may be used if none of them is feasible. |
How custom lentiviral production service proceeds:
If lentiviral construct is ready, please provide us:
1. The construct map or sequence.2.1~5 µg of your lentiviral construct plasmid DNA or bacterial culture.
We will prepare Endotoxin-free plasmid and transfect our packaging cell line for your lentivirus production. Harvest lentivirus particle followed by concentration and titration.
You will receive >200 ul of lentiviral vectors in 25 µl aliquots as indicated in the Table above.
If you need us to generate lentiviral vector for your target gene, please:
1. Choose lentiviral vector format from our vector collection and specify the gene of interest.
2. Please send us 1~5 µg plasmid or bacterial culture for your gene if it is available, otherwise send us cDNA sequence.
We will subclone the gene of interest or shRNA into chosen lentiviral vector and confirm by sequencing, prepare high quality plasmid DNA and transfect packaging cell line, harvest lentivirus particle followed by concentration and titration.
You will receive >200 ul of lentiviral vectors in 25 µl aliquots as indicated in the Table above.
For mini- or standard scale production, select the service below and add to your cart. For large scale production, complete the Request a Quote Form below.