Lentiviral sgRNA CRISPR

Premade sgRNA CRISPR/Cas9 lentivirus provides a convenient way for your gene knock-out study, especially gene knock-out in hard-to transfect cells.

CRISPR/Cas9 gene targeting requires a specific single guide RNA (sgRNA). The sgRNA is composed of a  ~20 nucleotide target specific sequence (crRNA) and a Cas9 nuclease-recruiting sequence (tracrRNA). The specific gene targeting can be achieved by simply changing the crRNA sequence in the gRNA. 

Our premade sgRNA CRISPR/Cas9 lentiviruses express sgRNA under the control of a human U6 promoter, Cas9 nuclease and puromycin selection marker under the control of the EF1a promoter. The sgRNA CRISPR/Cas9 cells can be rapidly selected by puromycin. 

The current premade sgRNA CRISPR/Cas9 lentivirus collection includes sgRNA for 606 genes involved in ubiquitination pathway. Those sgRNA lentiviruses can be purchased individually or together as an array. 

Please click on individual product to learn more and place your order.